ChemicalBook Product Catalog Chemical Reagents Organic reagents Sulfonates / Sulfinates salts Dansyl chloride

Dansyl chloride

Product NameDansyl chloride
CAS605-65-2
MFC12H12ClNO2S
MW269.75
EINECS210-092-6
MOL File605-65-2.mol

Chemical Properties

Melting point	72-74 °C(lit.)
Boiling point	371.3±22.0 °C(Predicted)
Density	1.1702 (rough estimate)
refractive index	1.5630 (estimate)
storage temp.	2-8°C
solubility	acetone: 50 mg/mL
form	powder and chunks
pka	2.38±0.40(Predicted)
color	yellow to orange
Appearance	Yellow solid
Water Solubility	Soluble in dimethyl fluoride, acetone, chloroform, pyridine, benzene and dioxane. Insoluble in water.
Sensitive	Moisture Sensitive
ex/em	λex 380 nm; λem 475 nm
Merck	14,2814
BRN	2217205
Stability	Hygroscopic, Moisture Sensitive
InChI	1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
InChIKey	XPDXVDYUQZHFPV-UHFFFAOYSA-N
SMILES	CN(C)c1cccc2c(cccc12)S(Cl)(=O)=O
CAS DataBase Reference	605-65-2(CAS DataBase Reference)
EPA Substance Registry System	1-Naphthalenesulfonyl chloride, 5-(dimethylamino)- (605-65-2)

Safety Information

Hazard Codes	C
Risk Statements	34
Safety Statements	26-36/37/39-45-3
RIDADR	UN 3261 8/PG 2
WGK Germany	3
RTECS	QK3688000
F	10-21
TSCA	TSCA listed
HazardClass	8
PackingGroup	III
HS Code	29214980
Storage Class	8A - Combustible corrosive hazardous materials
Hazard Classifications	Eye Dam. 1 Met. Corr. 1 Skin Corr. 1B

MSDS

Provider	Language
5-(Dimethylamino)naphthalene-1-sulfonyl chloride	English
SigmaAldrich	English
ACROS	English
ALFA	English

Usage And Synthesis

Uses

Dansyl chloride or 5-Dimethylaminonaphthalene-1-sulfonyl chloride (CAS 605-65-2) is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.
Dansyl chloride is non-fluorescent until it reacts with amines. The resulting Dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl chloride an important tool for biophysical studies.

Description

Dansyl Chloride is used for fluorescent labelling of amines, amino acids and proteins.

Chemical Properties

yellow to orange-yellow crystalline powder

Uses

A fluorogenic reagent for N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

Uses

Dansyl chloride is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.

Uses

In fluorescent labelling, column chromatography, HPLC.

Uses

Reagent for fluorescent labelling of amines, amino acids and proteins.

Definition

ChEBI: Dansyl chloride is an aminonaphthalene and a sulfonic acid derivative.

General Description

Dansyl chloride is a fluorogenic reagent which is typically employed for the derivatization of amino acids.

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins. Dansyl chloride is used for derivatization of free amino acids and amines in neural samples and biological fluids. It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.

Safety Profile

Poison by intravenous route, When heated to decomposition it emits very toxic fumes of Cland NOx

Dansyl chloride

Chemical Properties

Safety Information

MSDS

Usage And Synthesis

Preparation Products And Raw materials

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