Basic information Description Uses Safety Related Supplier
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Fluorescein isothiocyanate

Basic information Description Uses Safety Related Supplier
Fluorescein isothiocyanate Basic information
Fluorescein isothiocyanate Chemical Properties
Safety Information
MSDS
Fluorescein isothiocyanate Usage And Synthesis
  • DescriptionFluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
    • Excitation source: 488 nm spectral line, argon-ion laser
    • Excitation wavelength: 494 nm
    • Emission wavelength: 518 nm
    • Extinction coefficient: > 70,000 M-1cm-1
    • Solubility: Soluble in aqueous buffers at pH > 6
    • Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
  • Uses
    1. Label antibodies for use as immunofluorescent probes
    2. Label oligonucleotides for hybridization probes
    3. Detect proteins in gels and on Western blots
  • Chemical PropertiesDark yellow powder
  • UsesFor fluorescent labeling of proteins (e.g. immunofluorescence); protein labeling for gel chromatography.
Fluorescein isothiocyanate Preparation Products And Raw materials
Fluorescein isothiocyanate (27072-45-3)Related Product Information
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