ChemicalBook Product Catalog Analytical Chemistry Liquid Chromatography HPLC labeled reagent 5-Aminofluorescein

5-Aminofluorescein

Product Name5-Aminofluorescein
CAS3326-34-9
MFC20H13NO5
MW347.32
EINECS222-043-6
MOL File3326-34-9.mol

Chemical Properties

Melting point	223 °C (dec.)(lit.)
Boiling point	481.89°C (rough estimate)
Density	1.3724 (rough estimate)
refractive index	1.5180 (estimate)
storage temp.	room temp
solubility	DMF: 5 mg/ml; DMSO: 5 mg/ml
pka	9.34±0.20(Predicted)
form	powder
color	red-brown
Appearance	Solid Powder
Water Solubility	Soluble in water (partly), methanol (10 mg/ml), and acetone.
λmax	496 nm
BRN	48395
Stability	Light and Moisture Sensitive
Major Application	diagnostic assay manufacturing hematology histology
InChI	1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChIKey	GZAJOEGTZDUSKS-UHFFFAOYSA-N
SMILES	Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
CAS DataBase Reference	3326-34-9(CAS DataBase Reference)
EPA Substance Registry System	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9)

Safety Information

Hazard Codes	Xn
Risk Statements	22-36/37/38
Safety Statements	26
WGK Germany	3
F	10
TSCA	TSCA listed
HS Code	32049090
Storage Class	11 - Combustible Solids
Hazard Classifications	Acute Tox. 4 Oral Eye Irrit. 2 Skin Irrit. 2 STOT SE 3

MSDS

Provider	Language
5-Amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid	English
SigmaAldrich	English
ACROS	English

Usage And Synthesis

Description

5-AMF is a fluorescent probe for carboxylic acids and glutamine, and it also can be coupled to aldehydes and ketones.

Chemical Properties

Dark Red Solid

Uses

5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.

Uses

Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020).

Definition

ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form).

Preparation

The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.

General Description

Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.

Preparation Products And Raw materials

5-Aminofluorescein Supplier

5-Aminofluorescein manufacturers

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