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TRIS hydrochloride

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TRIS hydrochloride Basic information
TRIS hydrochloride Chemical Properties
  • Melting point:150-152 °C
  • Density 1.05 g/mL at 20 °C
  • storage temp. room temp
  • solubility H2O: 4 M at 20 °C, clear, colorless
  • form crystalline
  • color clear colorless (40 % (w/w) in H2O) solution
  • PH Range7.0 - 9.0
  • PH2.5-4.0 (25℃, 4M in H2O)
  • pka8.1(at 25℃)
  • Water Solubility SOLUBLE
  • Sensitive Hygroscopic
  • λmaxλ: 260 nm Amax: 0.12
    λ: 280 nm Amax: 0.08
  • BRN 3675235
  • Stability:Stable. Incompatible with strong oxidizing agents, bases, moisture.
  • InChIKeyQKNYBSVHEMOAJP-UHFFFAOYSA-N
  • CAS DataBase Reference1185-53-1(CAS DataBase Reference)
  • EPA Substance Registry System1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)
Safety Information
  • Hazard Codes Xi
  • Risk Statements 36/37/38
  • Safety Statements 26-36
  • WGK Germany 3
  • RTECS TY2900000
  • 3
  • TSCA Yes
  • HS Code 29221980
MSDS
TRIS hydrochloride Usage And Synthesis
  • Chemical PropertiesWhite crystalline powder
  • UsesFor precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode;For Tris buffers, pH increases about 0.03 unit/°C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
  • Biological ActivityCommonly used laboratory reagent
  • Purification MethodsCrystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]
TRIS hydrochloride(1185-53-1)Related Product Information
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