ChemicalBook > Product Catalog > Biochemical Engineering > Amino Acids and Derivatives > Glycine derivative > Tricine
Tricine
- Product Name:Tricine
- CAS:5704-04-1
- MF:C6H13NO5
- MW:179.17
- EINECS:227-193-6
- Mol File:5704-04-1.mol
-
Tricine Chemical Properties
- Melting point:186-188 °C (dec.)
- Boiling point:311.69°C (rough estimate)
- Density 1.05 g/mL at 20 °C
- refractive index 1.4240 (estimate)
- storage temp. Keep in dark place,Inert atmosphere,Room temperature
- solubility H2O: 4 M at 20 °C, clear, colorless
- form crystalline
- color clear colorless (40 % (w/w) in H2O) solution
- PH4.0-6.0 (1M in H2O)
- pka8.1(at 25℃)
- PH Range7.4 - 8.8
- OdorOdorless
- Water Solubility soluble
- Merck 14,9651
- BRN 1937804
- Stability:Stable.
- CAS DataBase Reference5704-04-1(CAS DataBase Reference)
- EPA Substance Registry SystemGlycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
- Hazard Codes Xi
- Risk Statements 36/37/38
- Safety Statements 26-36-36/37/39
- WGK Germany 3
- F 3
- TSCA Yes
- HS Code 29052900
- Language:EnglishProvider:SigmaAldrich
- Language:EnglishProvider:ACROS
- Language:EnglishProvider:ALFA
Tricine Usage And Synthesis
- DescriptioinTricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
- Reference
- S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
- H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
- Chemical PropertiesWhite/clear crystalline powder
- UsesGood's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets.
A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
A zwitterionic amino acid commonly useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C. - UsesTricine is a commonly used electrophoresis buffer.
- Biological ActivityElectrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
- Purification MethodsCrystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]
GlycineMax
Di(trimethylol propane)
Sarcosine
TRIS-TRICINE,TRIS-TRICINE ANODE BUFFER
CHLOROPHOSPHONAZO III
Methyl
2-(HYDROXYMETHYL)-1,3-PROPANEDIOL
TRICINE (D13)
N-TERT-BUTYLETHYLAMINE
2-(2-HYDROXYETHYLAMINO)-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL
2-Amino-2-methyl-1,3-propanediol
N-METHYL-TERT-BUTYLAMINE
PROTEOGEL(TM) TRIS-TRICINE-SDS ELECTRODE BUFFER,TRIS-TRICINE-SDS,TRIS-TRICINE-SDS BUFFER
Ethyl acetate
Tris Base
ISOXADIFEN-ETHYL
Sodium hydroxymethylglycinate
Kresoxim-methyl
TricineSupplierMore
- Company Name:Hunan Yunbang Biomedical Co., Ltd Gold
- Tel:13319523173 133-08453923-
- Email:ivy@hnhbsj.com;ivy@hnhbsj.com
- Company Name:Nanjing Search Biotech Co., Ltd Gold
- Tel:13515111588 025-81682922-
- Email:sales@searchbio.com.cn;sales@searchbio.com.cn
- Company Name:Energy Chemical
- Tel:400-005-6266 021-58432009-
- Email:sales8178@energy-chemical.com
- Company Name:BEST-REAGENT
- Tel:18981987031 400-1166-196
- Email:cdhxsj@163.com;1955352637@qq.com;cdhxsj@163.com
- Company Name:Hunan Hui Bai Shi Biotechnology Co., Ltd.
- Tel:13308475853 0731-85526065-
- Email:3002732518@qq.com
