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Basic information Descriptioin Reference Safety Related Supplier
Tricine Basic information
Tricine Chemical Properties
  • Melting point:186-188 °C (dec.)
  • Boiling point:311.69°C (rough estimate)
  • Density 1.05 g/mL at 20 °C
  • refractive index 1.4240 (estimate)
  • storage temp. Keep in dark place,Inert atmosphere,Room temperature
  • solubility H2O: 4 M at 20 °C, clear, colorless
  • form crystalline
  • color clear colorless (40 % (w/w) in H2O) solution
  • PH4.0-6.0 (1M in H2O)
  • pka8.1(at 25℃)
  • PH Range7.4 - 8.8
  • OdorOdorless
  • Water Solubility soluble
  • Merck 14,9651
  • BRN 1937804
  • Stability:Stable.
  • CAS DataBase Reference5704-04-1(CAS DataBase Reference)
  • EPA Substance Registry SystemGlycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
Safety Information
  • Hazard Codes Xi
  • Risk Statements 36/37/38
  • Safety Statements 26-36-36/37/39
  • WGK Germany 3
  • 3
  • TSCA Yes
  • HS Code 29052900
Tricine Usage And Synthesis
  • DescriptioinTricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
  • Reference
    1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
    2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
  • Chemical PropertiesWhite/clear crystalline powder
  • UsesGood's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets.
    A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
    A zwitterionic amino acid commonly useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
  • UsesTricine is a commonly used electrophoresis buffer.
  • Biological ActivityElectrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
  • Purification MethodsCrystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]
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