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Fluorexon

Basic information Safety Related Supplier
Fluorexon Basic information
Fluorexon Chemical Properties
  • Melting point:200 °C
  • Boiling point:662.7°C (rough estimate)
  • Density 1.4388 (rough estimate)
  • refractive index 1.5800 (estimate)
  • storage temp. Store at +5°C to +30°C.
  • solubility H2O: soluble
  • form Powder
  • pka6.67(at 25℃)
  • color Yellow-orange to orange-brown
  • PH RangeWeak green ' uorescence (6.0) to strong green ' uorescence (7.2)
  • Water Solubility insoluble
  • λmax494nm
  • Stability:Stable. Incompatible with strong oxidizing agents.
  • Biological ApplicationsCalcium indicator; fluoride indicator; iron indicator; mercury indicator; detecting nucleic acids,proteins; treating osteoporosis; drug delivery systems
  • Major ApplicationChemical-mechanical polishing, stabilizing liposomes, cell motility assay, detecting nucleic acids, virus, screening safety of gastric mucosa, MDR inhibitors, delivery, imaging atherosclerotic plaque
  • CAS DataBase Reference1461-15-0(CAS DataBase Reference)
  • EPA Substance Registry SystemGlycine, N,N'-[(3',6'- dihydroxy-3-oxospiro[isobenzofuran- 1(3H),9'-[9H]xanthene]- 2',7'-diyl)bis( methylene)]bis[N-(carboxymethyl)- (1461-15-0)
Safety Information
MSDS
Fluorexon Usage And Synthesis
  • Chemical Propertiesorange crystals
  • UsesCalcein is used for determination of Ca in biological systems. Indicator for the fluorimetric determination of calcium in presence of Mg by complexometric titration.
  • UsesCalcein is a anthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. [Cayman Chemical]
  • Purification MethodsDissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]
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