Bacillus thuringiensis

Bacillus thuringiensis or Bt is a naturally occurring rod-shaped, spore-forming, aerobic, grampositive micro-organism (bacterium) that is found throughout most areas of the world. It can be found in soils and on leaves/needles and in other common environmental situations. When the bacteria produces spores, it also produces unique crystalline proteins. When eaten, these natural proteins are toxic to certain insects, but not to human beings, birds, or other animals.

Bacillus thuringiensis is the most widely known and researched bacterium within this group and is differentiated from other spore-forming bacilli by the presence of a parasporal body that is formed within the sporangium during sporogenesis. The parasporal body is a high-molecular-mass protein crystal that is referred to as crystalline protein, δ-endotoxin, as well as a parasporal body. This protein moiety possesses some of the insecticidal properties of the bacterium.

Bacillus thuringiensis is commonly known as B.t. It is a microorganism that produces chem- icals toxic to insects. B.t. was registered in the United States for use as a pesticide in 1961 and re-registered in 1998. B.t. occurs naturally in the environment and has been isolated from soil, insects, and plant surfaces. B.t. pesticides are used for food and non-food crops, greenhouses, forests, and outdoor home use. B.t. pesticides exist in granular, powder, dust, suspension, and l owable forms. A number of insecticides are based on these toxins. B.t. is considered ideal for pest man- agement because of its specii city to pests and because of its lack of toxicity to humans as well as natural enemies of many crop pests. There are different strains of B.t., each with specii c toxicity to particular types of insects. For instance, B.t. aizawai (B.t.a.) is used against wax moth larvae in honeycombs, B.t. israelensis (B.t.i.) is effective against mos- quitoes, blackl ies and some midges, and B.t. kurstaki (B.t.k.) controls various types of lepidopterous insects, including the gypsy moth and cabbage looper. A new strain, B.t. san diego, has been found to be effective against certain beetle species and the boll weevil. In order to be effective, B.t. must be eaten by insects in the immature, feeding stage of larvae development. B.t. is ineffective against adult insects. Regular monitoring of the target insect population before application of B.t. ensures good control of the vulnerable larval stage. More than 150 insects, mostly lepidopterous larvae, are known to be suscep- tible in some way to B.t. Death of target larvae is known to occur within a few hours to a few weeks of B.t. application, depending on the species of insect and the amount of B.t. ingested by the insect. B.t. is moderately persistent in soil and its toxins degrade rapidly. The movement of B.t. is limited following pesticide application and it is unlikely to contaminate groundwater. B.t. is not native to water and is not likely to multiply in water.

Bacillus thuringiensis (Bt) is a bacterium that was first identified by S. Ishiwata in 1901 in Japanese silkworms presenting flacherie, or flaccid disease. It was later scientifically described and named by E. Berliner in Thüringen, Germany (Knowles 1994) . Berliner noted that the bacterium was producing insecticidal crystal (Cry) proteins, which were causing cell death in the digestive tract. These proteins have been used in insecticidal sprays and dusts to control pest insects since the 1930s. However, today they are most widely utilized as a transgene inserted into crop plants. These transgenic plants (Bt crops) are able to express the bacterial toxin-genes to defend themselves against herbivory. The use of these crops has been effective in controlling pest populations and increasing crop yield and quality. Bt crops were first commercialized in 1996 and, in 2013 187.5 million acres of Bt transgenic crops were planted worldwide (James et al. 2014).

Bt has been used in spray formulations for more than 40 years, and more recently its insecticidal protein genes have been incorporated into several major crops. Due to their insecticidal activity, Cry toxins are used worldwide as bioinsecticides to control disease-vector insect and crop pest populations.
One of the most successful applications of Bt has been the control of lepidopteran defoliators, which are pests of coniferous forests mainly in Canada and the United States. Bt subsp. israelensis (Bti) is highly active against larvae of disease-vector mosquitoes like Aedes aegypti (vector of dengue fever), Aedes albopictus (vector of chikungunya), Simulium damnosum (vector of onchocerciasis), and certain Anopheles species (vectors of malaria). Bti formulations (WG, water-dispersible granule; DT, ready-to-use tablet) have been evaluated by the World Health Organization Pesticide Evaluation Scheme (WHOPES) and recommended as mosquito larvicides, including their use against mosquito larvae that develop in drinking-water containers. Successful application of Bt is highly dependent on proper timing, weather conditions, and dosage of spray applications. These factors combine to determine the probability of larvae ingesting a lethal dose.
Recently, the use of Cry toxins has increased dramatically following the introduction of cry genes into plants. These ‘Bt crops’ have thus far proved to be an effective control strategy, and in 2004 Bt-maize and Bt-cotton were grown on 22.4 million hectares worldwide. Such widespread use, however, has led to concerns about the effect Bt crops may have on the environment and on human health.

These early experiments showed that Bt toxins needed to be activated in the gut, and it was soon discovered that the critical factors were an alkaline environment and the presence of specific proteases, which cleaved the innocuous protoxin into its active form. Once activated by proteolysis, each toxin binds to receptors in the brush border membrane and causes pores to open, disrupting them ovement of solutes across the gut epithelium and causing the influx of water. The toxins were shown to be orally lethal to caterpillars in pure form, and the pro-toxins could be converted into active toxinsin vitro, using specific pro-teases under alkaline conditions. The requirement for alkaline conditions, specific proteases and specific receptors explains why Bt is harmless to mammals (which have anacidic gut and lack the corresponding receptors) and why each toxin has a narrow host range.
 Nine common pests of rice, cotton and maize that are controlled by Bt crops

The insecticidal action of B.t. is attributed to protein crystals produced by the bacte- rium. Exposures of test animals to B.t. using several routes did not produce any acute toxicity in birds, dogs, guinea pigs, mice, or rats. Also laboratory rats when injected with B.t.k., showed no toxic or virus-like effects. No oral toxicity was found in rats, mice, or Japanese quail fed protein crystals from B.t. var. israelensis. Studies indicated that after rats ate B.t., the microorganism remained in the digestive system until it was eliminated from the body. Rabbits exposed to B.t. showed mild skin irritation and rats showed low inhalation toxicity to B.t. In fact, chronic toxicity studies in dogs, guinea pigs, rats, and other species of test animals showed no evidence of adverse health effects. The toxicity of B.t. is insect specii c. Researches have provided valuable data and identii ed B.t. subspecies that differ in toxicity to different insects. Examples of B.t. subspecies and the insects they affect are aizawai (moths), kurstaki (moths), israelen- sis (mosquitoes and l ies), and tenebrionis (beetles). Also, phytotoxicity studies (plant researches) showed B.t. genes in some crops (B.t. crops) to combat insects of corn crops, cotton, and potatoes. B.t. must be eaten by insects to be effective and works by interfer- ing with digestion. Insects are most sensitive to B.t. when they are larvae, an immature life stage. Insects that eat B.t., die from hunger or infection. It does not cause disease outbreaks in insect populations. B.t. may produce toxic chemicals that are released from the organism

Bacillus thuringiensis (Bt) is an important insect pathogenic bacterium commercially known as 'Thuricide' It releases toxic polypeptide crystals which are degradable by the enzyme, protease. The bacterium is pathogenic to the following insects: Lepidoptera, Diptera and Coleoptera.
Bacillus thuringiensis has been exploited commercially and its sprays have been used in the USA since the 1930s. It is the only commercialized transgene. The Bt toxin provides resistance against insects by binding to specific sites in the insect gut. However, insect resistance to Bt is also known.

Low toxicity by ingestion and skincontact. When heated to decomposition it emits acridsmoke and irritating vapors.

Bt Cry and Cyt toxins belong to a class of bacterial toxins known as pore-forming toxins (PFT) that are secreted as watersoluble proteins undergoing conformational changes in order to insert into, or to translocate across, cell membranes of their host. The primary action of Cry toxins is to lyse midgut epithelial cells in the target insect by forming pores in the apical microvilli membrane of the cells.
Bt is ineffective against adult insects and must be eaten by feeding larvae in order to be toxic. When ingested by insect larvae, sporulated-Bt crystalline inclusions dissolve in the alkaline environment of insect gut, and the solubilized inactive protoxins are converted into protease resistant active Cry and Cyt toxins. Toxin activation involves N-terminal, Cterminal, and intra-molecular cleavage. The activated Cry toxins are composed of three functional domains, a seven ahelices bundle that is involved in membrane insertion (domain I), and two b-sheet domains (domains II and III) involved in receptor interactions. Once activated, Cry toxins bind to specific receptors on the brush border membrane of the midgut epithelium columnar cells before inserting into the membrane. Toxin insertion leads to the formation of lytic pores in microvilli of apical membranes. Subsequently cell lysis and disruption of the midgut epithelium release the cell content providing the spores with a germinating medium leading to a severe septicemia and insect death.

Bt is moderately persistent in soil with a half-life of ca. 4 months.
Bt subsp. israelensis is often applied directly to water for the control of mosquitoes and blackflies. It has been demonstrated that the sedimentation of Bti is facilitated by adsorption onto particulate material. Bti can persist as long as 5 months in cold water, and adsorption to particulate matter in water facilitates persistence.
Bt is relatively short-lived on foliage due to rapid photodegradation. Its half-life under normal sunlight conditions is 3.8 h. In general, Bt loses 50% of its insecticide activity in 1-3 days after spraying. However, high toxicity toward mosquito larvae has been found in decaying leaf litter collected in several natural mosquito breeding sites in forested areas. From the toxic fraction of the leaf litter, B. cereus-like bacteria were isolated and further characterized as Bt subsp. israelensis using PCR (polymerase chain reaction) amplification of specific toxin genes. The anthropogenic origin of Bti was demonstrated by amplified fragment length polymorphism (AFLP) profile comparisons. Nevertheless, persistence of acute toxicity several months after Bti spraying remains exceptional, as this was only observed once in only one out of eight sampling sites. In this particular site, Bti spores and toxins may be protected from degradation by the vegetal matrix.