Coumarin 151 is yellow powder
Coumarin 151 is used to prepare peptidase substrates and for the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids.
7-Amino-4-(trifluoromethyl)coumarin is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. It is suitable as laser dye.
ChEBI: 7-amino-4-(trifluoromethyl)coumarin is a member of 7-aminocoumarins. It has a role as a fluorochrome.
Purify the coumarin by column chromatography on a C18 column, elute with acetonitrile/0.01M H2O/HCl (1:1), and recrystallise it from isopropanol. Alternatively, it is eluted from a silica gel column with CH2Cl2, or by extracting a CH2Cl2 solution (4g/L) with 1M aqueous NaOH (3 x 0.1L), followed by drying (MgSO4), filtration and evaporation. [Bissell J Org Chem 45 2283 1980, Zimmermann et al. Anal Biochem 70 258 1976.]
