CRF is a hypothalamic peptide that releases ACTH and endorphin from the anterior pituitary. It is also a neurotransmitter in the central nervous system, and is involved in autonomic and endocrine responses to stress.
The extract is purified by ion-exchange on CM-cellulose, desalted, evaporated and lyophilised. Then separate from impurities by gel filtration through Sephadex G-50. [Lande et al. Biochemical Preparations 13 45 1971, Esch et al. Biochem Biophys Res Commun 122 899 1984].
