4-MUG (6160-78-7) is a versatile fluorogenic substrate for ?-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3? This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm
Substrate for fluorometric assay of ?-D-galactosidase
It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium.
ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position.
Chiu et al. (2017), Measuring ?-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1
Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042
Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ?-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067
