ChemicalBook Product Catalog Dyes and Pigments dye basic dyes AURAMINE O

AURAMINE O

Product NameAURAMINE O
CAS2465-27-2
MFC17H22ClN3
MW303.83
EINECS219-567-2
MOL File2465-27-2.mol

Chemical Properties

Melting point	>250 °C (dec.)(lit.)
bulk density	430kg/m3
storage temp.	Inert atmosphere,Room Temperature
solubility	10g/l
Colour Index	41000
form	Powder/Solid
pka	9.8, 10.7(at 25℃)
color	Yellow to ochre-yellow
PH	6-7 (10g/l, H2O, 20℃)
PH Range	6.5
Water Solubility	Soluble in water or ethanol
λmax	370 nm, 432 nm
ε(extinction coefficient)	≥12000 at 249-255nm in water at 0.005g/L ≥15000 at 367-373nm in water at 0.005g/L ≥36000 at 429-435nm in water at 0.005g/L
BRN	4030061
Henry's Law Constant	3.5×10¹⁰ mol/(m³Pa) at 25℃, HSDB (2015)
Stability	Stable. Incompatible with strong oxidizing agents.
Biological Applications	Detecting bacteria; treatment of cancers,cardiovascular disease,diabetesassociated pain,mechanical allodynia,metabolic syndrome,protozoan infections in fish; targeted drug delivery; as fungal inhibitors
Major Application	diagnostic assay manufacturing hematology histology
InChI	1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
InChIKey	KSCQDDRPFHTIRL-UHFFFAOYSA-N
CAS DataBase Reference	2465-27-2(CAS DataBase Reference)
NIST Chemistry Reference	Aniline, 4,4'-(imidocarbonyl)bis-(n,n-dimethyl)-, hydrochloride(2465-27-2)
EPA Substance Registry System	Auramine, monohydrochloride (2465-27-2)

Safety Information

Hazard Codes	T,Xn
Risk Statements	22-24-40-20/21/22
Safety Statements	36/37-45-36-22
RIDADR	UN 2811 6.1/PG 3
WGK Germany	3
RTECS	BY3675000
TSCA	TSCA listed
HS Code	3204 13 00
HazardClass	6.1
PackingGroup	III
Storage Class	11 - Combustible Solids
Hazard Classifications	Acute Tox. 4 Oral Aquatic Chronic 2 Carc. 2 Eye Irrit. 2
Hazardous Substances Data	2465-27-2(Hazardous Substances Data)
Toxicity	LD50 orally in Rabbit: 1000 mg/kg

MSDS

Provider	Language
ACROS	English
SigmaAldrich	English

Usage And Synthesis

Chemical Properties

Auramine is a yellow crystalline powder or flaky material.

Chemical Properties

yellow powder

Uses

Certified for use by fluorescence microscopy in Churukian's modification of Truant's fluorescent method for acid fast bacilli on paraffin sections.

Uses

Fluorescent stain for acid-resistant bacteria in sputum

Definition

ChEBI: A hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Zieh Neelsen. It also can be used to make a fluorescent Schiff reagent.

General Description

Yellow flakes or powder.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

AURAMINE O is incompatible with strong oxidizing agents.

Hazard

Probable carcinogen.

Fire Hazard

Flash point data for AURAMINE O are not available. AURAMINE O is probably combustible.

Biological Activity

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Potential Exposure

Auramine is used industrially as a dye or dye intermediate for coloring textiles; paper, and leather. Also used as an antiseptic (a powerful antiseptic in ear and nose surgery, and treatment of gonorrhea) and fungicide. Human exposure to auramine occurs principally through skin absorption or inhalation of vapors. Low-level dermal exposure to the consumer may occur but would be limited to any migration of auramine from fabric, leather, or paper goods.

Shipping

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 Dyes, solid, toxic, n.o.s. or Dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Properties and Applications

TEST ITEMS	SPECIFICATION
APPEARANCE	DARK YELLOWPOWDER
SHADE	BRIGHT YELLOWISH
HEAT RESISTANCE	280 °C min
DENSITY	1.07 g/cm ³
WATER FASTNESS	4-5
LIGHT FASTNESS	1-2
BLEACHABILITY (OXIDATIVE)	4
BLEACHABILITY (REDUCTIVE)	3
WATER SOLUBILITY AT 20 °C	10 g/L min
WATER INSOLUBLE	1.0% max
MOISTURE	3.0% max
TINTING STRENGTH	100-105 %
WEIGHT METAL TOTAL	50ppm max

Purification Methods

It crystallises from EtOH as the hydrochloride and is very slightly soluble in CHCl3, UV: 434nm ( 370). The free base (carbinolamine) has m 136o (from *C6H6). [Goldacre & Phillips J Chem Soc 1724 1949, Conrad et al. Biochemistry 9 1540 1970, Beilstein 14 IV 256.]

Incompatibilities

Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

Waste Disposal

Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal. Incinerate in furnace with afterburner and scrubber.

AURAMINE O

Chemical Properties

Safety Information

MSDS

Usage And Synthesis

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