Bicinchoninic Acid Disodium Salt

Description

2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu⁺). BCA and Cu⁺ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu²⁺ is converted to Cu⁺ as a function of protein concentration under alkaline conditions.

Chemical Properties

White to light yellow powder.
IUPAC Name is disodium [2,2'-biquinoline]-4,4'-dicarboxylate

Uses

For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.

Uses

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
• It has been used for determining protein concentration.

Application

2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.