Bicinchoninic Acid Disodium Salt

Description

2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu⁺). BCA and Cu⁺ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu²⁺ is converted to Cu⁺ as a function of protein concentration under alkaline conditions.

Chemical Properties

White to light yellow powder

Uses

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
• It has been used for determining protein concentration.

Uses

For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.

Application

2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.