ChemicalBook Product Catalog Chemical Reagents Organic reagents Azo, diazo compound Bicinchoninic Acid Disodium Salt

Bicinchoninic Acid Disodium Salt

Product NameBicinchoninic Acid Disodium Salt
CAS979-88-4
MFC20H13N2NaO4
MW368.32
EINECS629-761-2
MOL File979-88-4.mol

Chemical Properties

Melting point	300℃
storage temp.	Inert atmosphere,2-8°C
solubility	0.5% sodium bicarbonate: soluble50mg/mL
form	Powder/Chunks
color	white to light yellow
Water Solubility	Soluble in water.
Stability	Hygrscopic
Major Application	diagnostic assay manufacturing hematology histology
InChI	InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;
InChIKey	AUPXFICLXPLHBB-UHFFFAOYSA-L
SMILES	C(C1=CC(C2=NC3=CC=CC=C3C(C(=O)O)=C2)=NC2=CC=CC=C12)(=O)O.[NaH]
CAS DataBase Reference	979-88-4(CAS DataBase Reference)
EPA Substance Registry System	[2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4)

Safety Information

Hazard Codes	Xn,Xi
Risk Statements	36/37-42-36/38-36/37/38-20/21/22
Safety Statements	22-26-36/37-45-36
WGK Germany	3
TSCA	TSCA listed
HS Code	29339900
Storage Class	11 - Combustible Solids
Hazard Classifications	Eye Irrit. 2 Skin Irrit. 2

MSDS

Provider	Language
SigmaAldrich	English
ALFA	English

Usage And Synthesis

Description

2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu⁺). BCA and Cu⁺ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu²⁺ is converted to Cu⁺ as a function of protein concentration under alkaline conditions.

Chemical Properties

White to light yellow powder.
IUPAC Name is disodium [2,2'-biquinoline]-4,4'-dicarboxylate

Uses

For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.

Uses

Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
It has been used for determining protein concentration.

Application

2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

References

[1] P.K. SMITH. Measurement of protein using bicinchoninic acid[J]. Analytical biochemistry, 1985, 150 1: Pages 76-85. DOI: 10.1016/0003-2697(85)90442-7
[2] BRENNER A.J. Harris E D. A Quantitative Test for Copper Using Bicinchoninic Acid[J]. Analytical biochemistry, 1995, 226 1: Pages 80-84. DOI: 10.1006/abio.1995.1194
[3] WALKER J M. The bicinchoninic acid (BCA) assay for protein quantitation.[J]. Methods in molecular biology, 1994, 32: 5-8. DOI: 10.1385/0-89603-268-x:5

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