Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I Price

Fluorescein isothiocyanate isomer I Chemical Properties,Usage,Production

• Description

  Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.

• Chemical Properties

  Orange-yellow cryst.

• Uses

  Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

• Uses

  Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

• Uses

  Fluorescein -5-isothiocyanate is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

• Uses

  Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens

• Definition

  ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

• General Description

  In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
  FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

• Purification Methods

  It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

Preparation Products And Raw materials

Fluorescein isothiocyanate isomer I Supplier

Global(306)Suppliers

View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

• 

  Fluorescein 5-Isothiocyanate (isomer I)

  $15.00-10.00/ KG

  99%+ HPLC

  Zhuozhou Wenxi import and Export Co., Ltd

  Inquiry
• 

  Fluorescein isothiocyanate isomer I

  $1.00/ kg

  98%

  Career Henan Chemical Co

  Inquiry

3326-32-7, Fluorescein isothiocyanate isomer IRelated Search