3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on

Bezeichnung:3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on

CAS-Nr27072-45-3

Englisch Name:Fluorescein isothiocyanate

CBNumberCB3173163

SummenformelC21H11NO5S

Molgewicht389.38

MOL-Datei27072-45-3.mol

Synonyma

3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on

3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on physikalisch-chemischer Eigenschaften

Schmelzpunkt	>360 °C(lit.)
storage temp.	-20°C
Löslichkeit	DMSO (Slightly), Methanol (Slightly)
Aggregatzustand	powder
Farbe	Brown to Dark Brown
Merck	14,4089
BRN	1407295
Stabilität	Moisture Sensitive
InChIKey	WBSWSUYBEIMVMI-UHFFFAOYSA-N
CAS Datenbank	27072-45-3(CAS DataBase Reference)
EPA chemische Informationen	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	42/43-36/37/38-20/21/22-42
S-Sätze:	22-36-26-24/25-45-36/37
WGK Germany	3
F	10
HS Code	29321900

Gefahreninformationscode (GHS)

Fluorescein isothiocyanate Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R42/43:Sensibilisierung durch Einatmen und Hautkontakt möglich.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
R42:Sensibilisierung durch Einatmen möglich.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
Chemische Eigenschaften
Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.
Verwenden
Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.
Verwenden
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.
Application
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
Definition
ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
synthetische
By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Fluorescein isothiocyanate Upstream-Materialien And Downstream Produkte

3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

27072-45-3, Fluorescein isothiocyanate Verwandte Suche:

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