83-07-8

Amber crystalline powder

4-aminoantipyrine is the most widely used analytical reagent for the estimation of phenol. It is used as a reagent for glucose determination in the presence of peroxidase and phenol. It is also used as indicator for trace phenol determinations in water.
Phenolic compounds were determined by buffering the sample to a pH of 10.0 and adding 4-aminoantipyrine to produce a yellow or amber colored complex in the presence of ferricyanide ion. The colour is intensified through extraction of the complex into chloroform. Measurement of this colour quantitatively determines the phenol concentration of the sample.

4-Aminoantipyrine is a metabolite of aminopyrine, having both analgesic and anti-inflammatory properties.
Coupling Reagent for Trinder’s reagents in colorimetric hydrogen peroxide detectionassays
Forms highly stable dyes by coupling with Trinder’s reagents in presence of Peroxidase and H2O2. Therefore suitable for use in test strip and solution diagnostics.

A reagent for glucose determination in the presence of peroxidase and phenol

Indicator for trace phenol determinations in water.

ChEBI: A pyrazolone, a member of the class of pyrazoles that is antipyrine substituted at C-4 by an amino group. It is a metabolite of aminopyrine and of metamizole.

synthesis of 4-aminoantipyrine: Antipyrine is nitrosated by sodium nitrite, reduced by ammonium bisulfite and ammonium sulfite, hydrolyzed by sulfuric acid, and finally neutralized with liquid ammonia to obtain 4-aminoantipyrine.

It crystallises from EtOH or EtOH/ether. [Beilstein 25 III/IV 3554.]