General Description
Orange-red to dark red powder. Water-soluble form of fluorescein. Odorless and almost tasteless. pH (5% solution) 7.8. May be sensitive to prolonged exposure to light and to heat.
Reactivity Profile
FLUORESCEIN, DISODIUM SALT(518-47-8) is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals .
Air & Water Reactions
Hygroscopic. Water soluble.
Fire Hazard
Flash point data for this chemical are not available; however, FLUORESCEIN, DISODIUM SALT is probably combustible.
Chemical Properties
Orange-red powder; odorless. Hygroscopic,
soluble in water; sparingly soluble in
alcohol.
Uses
A sodium salt of fluorescein
Uses
In examining subterranean waters. Serves to ascertain source of springs, connections between streams and sea, determining approx vol of water delivered by a spring, detecting source of contamination of drinking water, infiltration of soil with waste waters of factories. In externally applied drugs and cosmetics. Analytical reagent (protein label). Clinical reagent (immuno-histological stain, immuno-fluorescent label).
Veterinary Drugs and Treatments
Fluorescein sodium is a yellow water-soluble dye that fluoresces
under a Wood’s Lamp, but is plainly visible after binding to corneal
stroma through an ophthalmic examination light source. It is
used most commonly to delineate full thickness loss of corneal epithelium
indicating the presence of a corneal ulceration. In this instance
it will stain the corneal stroma. The epithelium is not stained
because its outer lipid cell membrane repels the stain. Descemet’s
membrane will not stain with fluorescein stain and this is used to
indicate descemetocele formation, an ocular emergency.
Fluorescein stain is applied to the precorneal tear film in dogs
and cats and the break-up of this stain with time, as observed
through a slit lamp biomicroscope using a cobalt blue light source,
is used to determine the tear film break-up time (normal 19 seconds),
an indicator of tear film quality.
Fluorescein stain is applied to the tear film of dogs to determine
patency of the nasolacrimal outflow system. The normal wait time
is 2 – 5 minutes in dogs and up to 10 minutes in cats. A positive test
indicates patency of the system. A negative test is not indicative of
disease as the test is negative in a large percentage of normal animals.
Fluorescein stain, then, can be added to irrigating solution to
flush the nasolacrimal system, making detecting the irrigation solution
at the nose more obvious during flushing of the system.
Properties and Applications
brilliant yellow. Soluble in water and ethanol (with strong green fluorescent), water-soluble good. In strong sulfuric acid with weak for fluorescence yellow, it will be diluted with yellow precipitation. Its water solution and sodium hydroxide with dark green fluorescence for brunet solution. Mainly used for sea of target that fluorescent, facilitate aerial reconnaissance search. And can use significantly for the exploration of fluorescent groundwater. Can also be used for medicine and cosmetics shading, rarely used for silk printing and dyeing things.
