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518-47-8

Supplier Related Products Identification Chemical Properties Safety Data Hazard Information Material Safety Data Sheet(MSDS) Spectrum Detail Well-known Reagent Company Product Information

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Identification

Name
Fluorescein disodium salt
CAS
518-47-8
Synonyms
ACID YELLOW 73
CALCOCID URANINE B-4315
CI 45350
CI NO 45350
D AND C YELLOW NO 8
FLUORESCEIN DISODIUM
FLUORESCEIN DISODIUM SALT
FLUORESCEIN DISODIUM SALT HYDRATE
FLUORESCEIN SODIUM
FLUORESCEIN, SODIUM DERIVATIVE, SODIUM SALT
FLUORESCEIN SODIUM SALT
FLUORESCEIN, WATER SOLUBLE
HIDACID URANINE
S NO 880
SODIUM FLUORESCEIN
URANIN
URANINE
URANINE AP
URANINE SS
URANINE, WATER SOLUBLE
EINECS(EC#)
208-253-0
Molecular Formula
C20H10Na2O5
MDL Number
MFCD00167039
Molecular Weight
376.27
MOL File
518-47-8.mol

Chemical Properties

Appearance
orange powder
Appearance
Orange-red powder; odorless. Hygroscopic, soluble in water; sparingly soluble in alcohol.
Melting point 
320 °C
mp 
320 °C
storage temp. 
Store at +5°C to +30°C.
solubility 
H2O: soluble1mg/mL
form 
Powder
Colour Index 
45350
pka
2.2, 4.4, 6.7(at 25℃)
color 
Rust-orange
Stability:
Stable.
PH Range
Pink uorescence (4.0) to green uorescence (6.0)
PH
8.3 (10g/l, H2O, 20℃)
Odor
Odorless
Water Solubility 
500 g/L (20 ºC)
λmax
491nm, 493.5nm, 490nm
Merck 
14,4159
BRN 
3833041
Major Application
Nanolithography, nanoparticles, thin films, fuel cells, inks, laser, colored bubbles, cleansing products, identifying leaks in coolant, suntanning agent, hair dyes, cosmetics, dermatology, dental material, diagnosis of diabetic retinopathy, antitumor agent
InChIKey
NJDNXYGOVLYJHP-UHFFFAOYSA-L
CAS DataBase Reference
518-47-8(CAS DataBase Reference)
EPA Substance Registry System
518-47-8(EPA Substance)

Safety Data

Hazard Codes 
Xi
Risk Statements 
R36:Irritating to the eyes.
R36/37/38:Irritating to eyes, respiratory system and skin .
Safety Statements 
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S27:Take off immediately all contaminated clothing .
WGK Germany 
1

RTECS 
LM5425000


3
TSCA 
Yes
HS Code 
32041200
Safety Profile
Moderately toxic by intraperitoneal route. Mildly toxic by ingestion. Human systemic effects by intravenous route: arrhythmias, eye hemorrhage, nausea or vomiting. Experimental reproductive effects. Questionable carcinogen with experimental tumorigenic data. Mutation data reported. When heated to decomposition it emits toxic fumes of Na2O.
Hazardous Substances Data
518-47-8(Hazardous Substances Data)
Toxicity
LD50 in mice, rats (mg/kg): 4738, 6721 orally (Yankel, Loux)

Hazard Information

General Description
Orange-red to dark red powder. Water-soluble form of fluorescein. Odorless and almost tasteless. pH (5% solution) 7.8. May be sensitive to prolonged exposure to light and to heat.
Reactivity Profile
FLUORESCEIN, DISODIUM SALT(518-47-8) is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals .
Air & Water Reactions
Hygroscopic. Water soluble.
Fire Hazard
Flash point data for this chemical are not available; however, FLUORESCEIN, DISODIUM SALT is probably combustible.
Chemical Properties
Orange-red powder; odorless. Hygroscopic, soluble in water; sparingly soluble in alcohol.
Uses
In examining subterranean waters. Serves to ascertain source of springs, connections between streams and sea, determining approx vol of water delivered by a spring, detecting source of contamination of drinking water, infiltration of soil with waste waters of factories. In externally applied drugs and cosmetics. Analytical reagent (protein label). Clinical reagent (immuno-histological stain, immuno-fluorescent label).
Uses
A sodium salt of fluorescein
Veterinary Drugs and Treatments
Fluorescein sodium is a yellow water-soluble dye that fluoresces under a Wood’s Lamp, but is plainly visible after binding to corneal stroma through an ophthalmic examination light source. It is used most commonly to delineate full thickness loss of corneal epithelium indicating the presence of a corneal ulceration. In this instance it will stain the corneal stroma. The epithelium is not stained because its outer lipid cell membrane repels the stain. Descemet’s membrane will not stain with fluorescein stain and this is used to indicate descemetocele formation, an ocular emergency.
Fluorescein stain is applied to the precorneal tear film in dogs and cats and the break-up of this stain with time, as observed through a slit lamp biomicroscope using a cobalt blue light source, is used to determine the tear film break-up time (normal 19 seconds), an indicator of tear film quality.
Fluorescein stain is applied to the tear film of dogs to determine patency of the nasolacrimal outflow system. The normal wait time is 2 – 5 minutes in dogs and up to 10 minutes in cats. A positive test indicates patency of the system. A negative test is not indicative of disease as the test is negative in a large percentage of normal animals. Fluorescein stain, then, can be added to irrigating solution to flush the nasolacrimal system, making detecting the irrigation solution at the nose more obvious during flushing of the system.
Properties and Applications
brilliant yellow. Soluble in water and ethanol (with strong green fluorescent), water-soluble good. In strong sulfuric acid with weak for fluorescence yellow, it will be diluted with yellow precipitation. Its water solution and sodium hydroxide with dark green fluorescence for brunet solution. Mainly used for sea of target that fluorescent, facilitate aerial reconnaissance search. And can use significantly for the exploration of fluorescent groundwater. Can also be used for medicine and cosmetics shading, rarely used for silk printing and dyeing things.

Material Safety Data Sheet(MSDS)

msds information
C.I. 45350(518-47-8).msds

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