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Photobiotin acetate salt is a photo-activatable biotin analogue. It can be used in biological study and use for protein patterning with a photoactivatable derivative of biotin.

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.