76-54-0

Name	2',7'-DICHLOROFLUORESCEIN
CAS	76-54-0
EINECS(EC#)	200-968-6
Molecular Formula	C20H10Cl2O5
MDL Number	MFCD00005047
Molecular Weight	401.2
MOL File	76-54-0.mol

Synonyms

2',7'-DICHLORO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-[9H]XANTHEN]-3-ONE 2',7'-DICHLORO-3,6-FLUORANDIOL 2',7'-DICHLOROFLUORESCEIN 2,7-DICHLOROFLUORESCEIN 2',7'-DICHLORO-FLUORESCEINE 3,6-DICHLOROFLUORAN DICHLOROFLUORESCEIN DICHLOROFLUORESCEIN TS DICHLORO (R) FLUORESCEIN FLUORESCEIN 27 FLUORESCEIN DICHLORIDE TIMTEC-BB SBB008847 2’,t-dichlorofluorescein 9’-[9H]xanthen]-3-one,2’,7’-dichloro-3’,6’-dihydroxy-Spiro[isobenzofuran-1(3H) dcorange25(fluorescein dcorange25(fluorescein548) spiro[isobenzofuran-1(3h),9’-[9h]xanthen]-3-one,2’,7’-dichloro-3’,6’-dihydroxy 2-(2,7-dichloro-6-hydroxy-3-oxo-3-H-xanthen-9-yl)benzoic acid 2,7-Dichorofluorescin 2',7'-Dichlorofluorofluorescein

Chemical Properties

Appearance	red to yellow solid
Melting point	280 °C (dec.)(lit.)
Boiling point	514.77°C (rough estimate)
density	0.79 g/mL at 20 °C
refractive index	1.5300 (estimate)
Fp	12°C (53.6°F)
storage temp.	room temp
solubility	alcohol: passes test
form	crystalline
pka	4.46(at 25℃)
color	orange to red-brown, powder
PH Range	4.0 (colorless) - 6.6 (green)
Stability:	Stable. Incompatible with strong oxidizing agents.
Water Solubility	Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
λmax	509nm
BRN	58009
Major Application	Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
InChIKey	NZVMJBGGAQSNLI-UHFFFAOYSA-N
CAS DataBase Reference	76-54-0(CAS DataBase Reference)
EPA Substance Registry System	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0)

Safety Data

Hazard Codes	Xi
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . R36:Irritating to the eyes. less more
Safety Statements	S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . less more
RIDADR	UN 1219 3/PG 2
WGK Germany	3
RTECS	NT8050000
TSCA	Yes
HS Code	29321900

Hazard Information

Chemical Properties

red to yellow solid

Uses

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.

Uses

Widely used probe, e.g. for determination of carbohydrates by fluorescence densitometry after TLC and determination of H2O2 by chemiluminescence. Fluorescent additives used in indirect fluorimetric detection in OTC-HPLC.

Application

2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Definition

2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.

Biological Activity

2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

Biochem/physiol Actions

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

storage

Store at -20°C

References

[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.

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Chemical Properties

Safety Data

Hazard Information

Chemical Properties

Uses

Uses

Uses

Application

Definition

Biological Activity

Biochem/physiol Actions

storage

References

Questions And Answer

Note

Spectrum Detail

Well-known Reagent Company Product Information

Supplier

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