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596-09-8

Name FLUORESCEIN DIACETATE
CAS 596-09-8
EINECS(EC#) 209-877-6
Molecular Formula C24H16O7
MDL Number MFCD00005062
Molecular Weight 416.38
MOL File 596-09-8.mol

Chemical Properties

Appearance Light yellow powder
Melting point  200-203 °C(lit.)
Boiling point  494.73°C (rough estimate)
density  1.2738 (rough estimate)
refractive index  1.6810 (estimate)
storage temp.  −20°C
solubility  acetone: soluble25mg/mL
form  Crystalline Powder
pka 6.4(at 25℃)
color  Light yellow
PH Range Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility  Soluble in chloroform, DMSO, ethanol, and water.
λmax 490nm
BRN  62575
Biological Applications Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
Major Application LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
CAS DataBase Reference 596-09-8(CAS DataBase Reference)
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8)

Safety Data

Hazard Codes  Xi
Risk Statements 
R36/37/38:Irritating to eyes, respiratory system and skin .
Safety Statements 
S24/25:Avoid contact with skin and eyes .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S27:Take off immediately all contaminated clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
WGK Germany  3
TSCA  Yes
HS Code  32129000

Hazard Information

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