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596-09-8

供应商 相关产品 基本信息 物理化学性质 应用领域 安全数据 图谱信息 知名试剂公司产品信息 荧光素二乙酸盐价格(试剂级) 常见问题列表

产品图片

基本信息

中文
荧光素二乙酸盐
英文名称
3,6-DIACETOXYFLUORAN
DIACETYLFLUORESCEIN
DI-O-ACETYLFLUORESCEIN
FDA
FDA [FLUORESCEIN DIACETATE]
FLUORESCEIN DIACETATE
FLUORESCEIN DI-O-ACETATE
SUBSTRATE FDA ESTERASE
3’,6’-diacetylfluorescein
9’-[9h]xanthen]-3-one,3’,6’-bis(acetyloxy)-spiro[isobenzofuran-1(3h
9’-[9H]xanthen]-3-one,3’,6’-bis(acetyloxy)-Spiro[isobenzofuran-1(3H)
fluoresceinacetate
3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl diacetate
FLUORESCEIN DIACETATE, FOR FLUORESCENCE
-Cellstain-Fda
Fluorescein diacetate, pure, 97%
Fluoresceindi-O-acetate(FDA)
Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-bis(acetyloxy)-
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
Fluorescein diacetate, 97%, pure
CAS
596-09-8
中文名称
荧光素二乙酸酯
荧光素二乙酸盐
二乙酸荧光素
醋酸荧光素99%
荧光胺双乙酸盐
二乙酸螢光素酯
EINECS 编号
209-877-6
分子式
C24H16O7
MDL 编号
MFCD00005062
分子量
416.38
MOL 文件
596-09-8.mol

物理化学性质

外观性质
白色晶体粉末 ,活细胞染色。熔点:200-203 °C
熔点 
200-203 °C(lit.)

储存条件 
−20°C

BRN 
62575

应用领域

用途一
也叫做FDA,可透过细胞膜并作为荧光素在活细胞内积累。由于荧光素较BCECF或钙黄绿素的亲水性低,荧光素从细胞中渗漏的量也高。FDA也用于流式细胞仪。荧光素的激发和发射波长分别为488nm和530nm。
用途二
FDA可透过细胞膜并作为荧光素积蓄在活细胞内。由于荧光素较BCECF或Calcein的亲水性低,因此荧光素从细胞中渗漏的量也高。FDA也可用于流式细胞仪。荧光素的激发和发射波长分别为488 nm和530 nm。

安全数据

安全说明 
S24/25
WGK Germany 
3

海关编码
32129000

图谱信息

知名试剂公司产品信息

荧光素二乙酸盐价格(试剂级)

荧光素二乙酸盐价格(试剂级)
更新日期产品编号产品名称包装价格
2021/09/07F0240荧光素二乙酸酯
Fluorescein Diacetate
5G580元
2021/02/25C19166荧光素二乙酸盐
Fluorescein diacetate, 97%, pure
5g494元
2021/02/25C19166荧光素二乙酸盐
Fluorescein diacetate, 97%, pure
25g1783元

常见问题列表

生物活性
Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。
体外研究

Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC 50 s of 3.3±0.5 μM and 0.61±0.04 μM, respectively.


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