Chemical Properties
red to maroon crystalline solid
Definition
ChEBI: An organic sodium salt resulting from the formal condensation of Amido Black 10B (acid form) with two equivalents of sodium hydroxide.
General Description
Dark brown granular solid.
Air & Water Reactions
Can be explosive when as a dust in air at certain concentrations. Moderately soluble in water.
Reactivity Profile
Acid Black 1 may form toxic gases with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. May form flammable gases with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Reacts with iron to give a weaker, greener color .
Fire Hazard
Flash point data for Acid Black 1 are not available; however, Acid Black 1 is probably combustible.
Uses
Amido Black 10B is used as a histological, protein, and nucleic acid dye used in electrophoresis. Naphthol Blue Black is an important and commonly used industrial dye. This dye is also used in rapid staining of protein bands on nitrocellulose membranes such as Western blots. Naphthol Blue Black can be utilized as a nucleic acid stain in SDS-Page gels. Additionally this compound has been untilized in cell proliferation assays and histochemical studies. It is also used in criminal investigations to detect blood present with latent fingerprints. They are also used in paints, inks, plastics and leather.
Preparation
4-Nitrobenzenamine diazo, in acid conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid?coupling, aniline diazo, in alkaline conditions and formation of single accidentally coupled nitrogen compounds.
Flammability and Explosibility
Nonflammable
Biological Activity
Naphthol blue black dye can be used to stain proteins on polyacrylamide gelsagarose gels and nitrocellulose membranes. After electrophoresisfixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% th at of Coomassie Blue R.
Properties and Applications
blue and black. Dark brown powder. A blue-black soluble in water, soluble in ethanol is blue, slightly soluble in acetone, insoluble in other organic solvents. Meet strong sulfuric acid is blue light green, the bright blue light generated after diluted precipitation; Nitric acid solution in a dark after Red sauce for change, and it will generate dark green light blue after diluted precipitation; Nitric acid solution in a dark red to sauce; 10% sodium hydroxide solution in a bright blue. It with strong hydrochloric acid aqueous solution of the generation of green light blue precipitation; Add sodium hydroxide solution generation blue precipitation. In dyeing copper and iron ion, its colour and lustre is the former micro change, the latter slightly with green light. Even dye the gender is poorer. Mainly used for wool, silk, polyamide fiber and its blended fabric dyeing and printing, also can be used for paper, electrical-controlled aluminum, soap, wood, biological, leather, medicine and cosmetics shading, also can be used in the manufacture of ink.
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Standard
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Light Fastness
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Soaping
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Persperation Fastness
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Oxygen bleaching
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Fastness to seawater
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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ISO
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5
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AATCC
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7
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