(S)-(-)-1-(4-PYRIDYL)ETHANOL

1. Used for the synthesis of chiral 1-(4-pyridinyl)ethylamines
2. Precursor for intermediates of highly stable chiral (A)6-B Supramolecular Copolymers

1122-54-9

54656-96-1

The general procedure for the synthesis of (S)-4-pyridine-1-ethanol from 4-acetylpyridine was as follows: first, the relative activities of the 26 substrates were assessed by a previously described assay protocol and the enzyme-to-substrate concentration ratio was adjusted. In this procedure, the activity of α-chloroacetophenone was used as a benchmark (set at 100%). Subsequently, the reduction reaction of aromatic ketones was determined enantioselectively using a NADH regeneration system consisting of KcDH purified from Bacillus subtilis CGMCC 1.1398 and glucose dehydrogenase (GDH). The specific reaction conditions were as follows: 0.5 mM NAD+, 10 mM ketone substrate, 1 U KcDH, 50 mg glucose, and 2 U GDH were added to 1 mL of 50 mM potassium phosphate buffer (pH 7.0). the reaction was carried out for 16 h. After the reaction was carried out, the reaction mixture was homogenized into two parts. One portion was terminated by adding an equal volume of methanol and subsequently analyzed by HPLC to determine the conversion; the other portion was extracted with ethyl acetate and further analyzed for enantiomeric excess (ee). The chiral products were analyzed by HPLC or GC methods, and the specific operating conditions are detailed in Supplementary Table S1.