Experimentally, DL-ornithine was obtained from L-arginine by one-pot hydrolysis-extinction reaction in alkaline solution, and then directly bioconverted by lysine decarboxylase from HafniaalveiAS1.1009, D-ornithine hydrochloride was prepared in 45.3% yield, and putrescine was also obtained in 41.5% yield. The reaction of L-arginine to DL-ornithine was catalyzed by salicylaldehyde at 0.10 molar ratio under reflux condition with 1.0 mol/L sodium hydroxide aqueous solution in 3 h. The results of the study on the properties of lysine decarboxylase in the bioconversion showed that the addition of 1 mmol/L Fe2+ could increase the specific enzyme activity to 6 119 U. Under the optimized conditions, the conversion time was 16 h, which was suitable for preparing D-ornithine hydrochloride and putrescine. -ornithine hydrochloride and putrescine.
