ACYL-COENZYME A SYNTHETASE

Product NameACYL-COENZYME A SYNTHETASE
CAS9013-18-7
MF
MW0
EINECS232-747-5
MOL FileMol File

Chemical Properties

storage temp.	-20°C
form	powder
color	white
Specific Activity	≥2units/mg protein

Safety Information

Safety Statements	24/25
WGK Germany	3
F	10
HS Code	35079090
Storage Class	11 - Combustible Solids

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Chemical Properties

White powder

Uses

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:

as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
in in vitro fatty acylation assays
in the synthesis of (¹⁴C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (³H)Palmitate-CoA

General Description

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Biochem/physiol Actions

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .

Purification Methods

Purify the synthase by extraction with sucrose/HCO3 buffer, protamine sulfate precipitation, (NH4)2SO4 (66-65%) fractination (pH 4.35) and a second (NH4)2SO4 (35-60%) fractionation (pH 4.35). It has Km 0.15mM (Vrel 1.0) for octanoate and 0.41mM (Vrel 2.37) for heptanoate. The Km for ATP is 0.5mM, all at pH 9.0 in ethylene glycol buffer at 38o. [Jencks et al. J Biol Chem 204 453 1953, Methods Enzymol 5 467 1962.]