Chlorophyllin

The silkworm sand or green tea leaves at 40 a 50 drying, research fine powder, add 3 times the amount of powder ethanol, acetone mixture ( 1:1 ) at 40 a 50 extraction 1.5h, filtration, filtrate residue with the same volume of ethanol, acetone mixture and then extracted once. Combined 2 times the extract with sodium hydroxide pH 11, heating ( 50 ) saponification for 30 min. saponification after distillation and concentration recovery mixture ( 60 ) to the volume of the original 1/4 a 1/3, and then extracted with petroleum ether 4 times. The lower layer was adjusted to pH 7 with hydrochloric acid, adjusted to pH 2 with copper sulfate, and copper generation at 50 for 1h, left to cool, granular precipitate was formed. The precipitate was collected at room temperature, washed with 50 a 60C water, washed with 30% a 40% ethanol until the ethanol layer was light green, and then washed with petroleum ether until the oil layer was light green. The filter cake was dissolved with acetone and precipitated with 5% sodium hydroxide ethanol solution, pH 12, the precipitate was collected and washed with anhydrous ethanol to obtain copper chlorophyll sodium salt.