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CHLOROPHYLL A Basic information
CHLOROPHYLL A Chemical Properties
  • Melting point:117-120°
  • alpha D20 -262° (acetone)
  • storage temp. 2-8°C
  • solubility diethyl ether: soluble0.1mg/10 mL
  • form powder
  • Merck 13,2174
  • BRN 3586237
  • Stability:Stable, but may discolour upon exposure to light. Incompatible with strong oxidizing agents. May be air sensitive.
  • EPA Substance Registry SystemChlorophyll a (479-61-8)
Safety Information
  • Safety Statements 22-24/25
  • WGK Germany 3
  • RTECS FW6420000
  • 8-10-23
  • HS Code 29339900
CHLOROPHYLL A Usage And Synthesis
  • Chemical Propertiessolid
  • UsesChlorophyll A is a photosynthetic pigment that is essential for photosynthesis of eukaryotes and cyanobacteria, acting as a primary donor in the electron transport chain.
  • DefinitionOccurs in all photosynthetic organisms and is the major species in all of those that evolve oxygen.
  • Purification MethodsIt forms green crystals from Me2CO, Et2O/H2O, Et2O/hexane/H2O or Et2O/pentane /H2O. It is sparingly soluble in MeOH and insoluble in pet ether. In alkaline solution it gives a blue-green colour with deep red fluorescence. A very crude chlorophyll mixture has been purified by chromatography on low melting polyethylene (MI 0.044; “Dow” melting index MI <2) and developed with 70% aqueous Me2CO. The order of effluent from the bottom of the column is: xanthophylls, chlorophyll b , chlorophyll a, phaeophytins and carotenes. A mixture of chlorophylls a and b is best separated by chromatography on sugar, and the order is chlorophyll b elutes first followed by chlorophyll a. To an Me2CO/H2O solution of chlorophylls 200mL of iso-octane are added, and the mixture shaken in a separating funnel and the H2O is carefully removed. The iso-octane layer is dried (Na2SO4) and applied onto a glass column (5cm diameter) dry packed with 1000mL of powdered sucrose which has been washed with 250mL of iso-octane. Elution with 0.5% of isopropanol in iso-octane gives chlorophyll a. Keeping the eluate overnight at 0o yields micro crystals which are collected by filtration or centrifugation (Yield 40mg). UVEtOH has max 660, 613, 577, 531, 498, 429 and 409 nm. Note that anhydrous chlorophylls can be easily enolized, epimerized or allomerized in dry polar organic solvents. [Anderson & Calvin Nature 194 285 1962, Stoll & Weidemann Helv Chim Acta 16 739 757 1933, NMR: Katz et al. J Am Chem Soc 90 6841 1968, 85 3809 1963 for a and b; ORD: Inhoffen et al. Justus Liebigs Ann Chem 704 208 1967, Willst.tter & Isler Justus Liebigs Ann Chem 390 269, 233 1912, Beilstein 26 III/IV 3243.]
CHLOROPHYLL A Preparation Products And Raw materials
CHLOROPHYLL A(479-61-8)Related Product Information
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