Chlorophyllin

Description

Chlorophyllin are a group of water-soluble salts being semi-synthetic derivative of chlorophyll. Its major form sodium/copper derivative can be used as a food additive and in alternative medicine. As alternative medicine, it can be used as an aid to reduce the odor of urines or feces (bowel movements). It can also be used to improve the life quality in people who have fecal incontinence due to spinal cord injury, bowel cancer, psychotic disorder, terminal illness, or other disorders. It can also be sold as an herbal supplement. Chlorophyllin can also bind to mutagenic substances such as polycyclic aromatic benzo[a]pyrene and dibenzo{a,i}pyrene. As a topical preparation, it is useful for both treatment and odor control of wounds, injuries, and other skin conditions such as radiation burns. 

References

https://en.wikipedia.org/wiki/Chlorophyllin
http://www.everydayhealth.com/drugs/chlorophyllin

Chemical Properties

Dark green solid

Uses

Chlorophyllin, coppered trisodium salt is used in the spectrophotometric determination of Ven- lafaxine hydrochloride. A hybrid photoelectrochemical biofuel cell is composed of a TiO 2 film conductive glass electrode sensitized by a copper chlorophyll trisodium salt. Visible light-sensitive gels were prepared by introducing a chromophore (trisodium salt of copper chlorophyllin) to thermosensitive gels.

Biological Activity

Chlorophyllin is a water soluble derivative of chlorophyll. It has chemopreventive properties and forms a non-covalent complex with many mutagenic/carcinogenic molecules. The complex formation reduces absorption or allows excretion of the mutagenic/carcinogenic molecules. Chlorophyllin also exhibits antioxidant, antiapoptotic and immunostimulatory effects. It provides benefits, such as prevention of body odor and increased wound healing.

Synthesis

The silkworm sand or green tea leaves at 40 a 50 drying, research fine powder, add 3 times the amount of powder ethanol, acetone mixture ( 1:1 ) at 40 a 50 extraction 1.5h, filtration, filtrate residue with the same volume of ethanol, acetone mixture and then extracted once. Combined 2 times the extract with sodium hydroxide pH 11, heating ( 50 ) saponification for 30 min. saponification after distillation and concentration recovery mixture ( 60 ) to the volume of the original 1/4 a 1/3, and then extracted with petroleum ether 4 times. The lower layer was adjusted to pH 7 with hydrochloric acid, adjusted to pH 2 with copper sulfate, and copper generation at 50 for 1h, left to cool, granular precipitate was formed. The precipitate was collected at room temperature, washed with 50 a 60C water, washed with 30% a 40% ethanol until the ethanol layer was light green, and then washed with petroleum ether until the oil layer was light green. The filter cake was dissolved with acetone and precipitated with 5% sodium hydroxide ethanol solution, pH 12, the precipitate was collected and washed with anhydrous ethanol to obtain copper chlorophyll sodium salt.