D-荧光素钾盐
D-荧光素钾盐 性质
| 储存条件 | Inert atmosphere,Store in freezer, under -20°C |
|---|---|
| 溶解度 | DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM) DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM) Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM) PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM) Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM) |
| 形态 | 结晶固体 |
| 颜色 | 浅黄色 |
| BRN | 5416262 |
| InChI | InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3 |
| InChIKey | UMBKGTQQGYPQBE-OGFXRTJISA-M |
| SMILES | C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r| |
| CAS 数据库 | 115144-35-9(CAS DataBase Reference) |
D-荧光素钾盐 用途与合成方法
D-荧光素钾盐是荧光素酶的水溶性底物，存在于多种发光生物体中。在ATP和荧光素酶的催化作用下，D-荧光素钾被氧化，产生蓝绿色的光(560nm)，当底物过量时，产生的光量子数与荧光素酶的浓度呈正相关。编码荧光素酶的Luc基因是植物、哺乳动物细胞的常用报告基因。由于没有背景干扰，因此可以很容易地检测出低至0.02 pg 水平的荧光素酶。
D-荧光素钾盐是一类在生物体中发现的能引起生物发光的杂环化合物，如萤火虫。在ATP存在下，萤光素酶将其氧化脱羧后会发光。化学研究中可用于荧光素酶的基板。
D-Luciferin是萤火虫荧光素酶的底物。
D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly
Photinus pyralis
, the enzyme more frequently used in techniques of in vitro and optical imaging.
D-Luciferin exhibits a decrease in the measured K
m
in PC3M-Luc cell lysates with a K
m
of 34 μM.
Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection.
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H
2
O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.
D-荧光素钾盐 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-11 | XW1151443592 | D-荧光素钾盐 | 115144-35-9 | 5MG | 42 |
| 2024-11-11 | XW1151443591 | D-荧光素钾盐 | 115144-35-9 | 1MG | 33 |
