D-荧光素钾盐

简介用途生物活性体外研究体内研究 D-荧光素钾盐试剂级价格

D-荧光素钾盐

CAS号:115144-35-9
英文名:D-Luciferin potassium salt
中文名:D-荧光素钾盐
CBNumber:CB3740228
分子式:C11H9KN2O3S2
分子量:320.42
MOL File:115144-35-9.mol

D-荧光素钾盐化学性质

储存条件 :Inert atmosphere,Store in freezer, under -20°C
溶解度 :DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM)
DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM)
Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM)
PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM)
Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM)
形态 :A crystalline solid
颜色 :Light yellow
BRN :5416262
InChI :InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3
InChIKey :UMBKGTQQGYPQBE-OGFXRTJISA-M
SMILES :C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r|
CAS 数据库 :115144-35-9(CAS DataBase Reference)

安全信息

WGK Germany :3
海关编码 :29342000

D-荧光素钾盐性质、用途与生产工艺

简介
D-荧光素钾盐是荧光素酶的水溶性底物，存在于多种发光生物体中。在ATP和荧光素酶的催化作用下，D-荧光素钾被氧化，产生蓝绿色的光(560nm)，当底物过量时，产生的光量子数与荧光素酶的浓度呈正相关。编码荧光素酶的Luc基因是植物、哺乳动物细胞的常用报告基因。由于没有背景干扰，因此可以很容易地检测出低至0.02 pg 水平的荧光素酶。
用途
D-荧光素钾盐是一类在生物体中发现的能引起生物发光的杂环化合物，如萤火虫。在ATP存在下，萤光素酶将其氧化脱羧后会发光。化学研究中可用于荧光素酶的基板。
生物活性 D-Luciferin是萤火虫荧光素酶的底物。
体外研究

D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis , the enzyme more frequently used in techniques of in vitro and optical imaging.
D-Luciferin exhibits a decrease in the measured K _m in PC3M-Luc cell lysates with a K _m of 34 μM.
体内研究

Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection.
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H ₂ O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.
用途活细胞、组织或生物体内luc标记基因和荧光素酶-融合基因体内/体外表达的成像分析； 2）广泛用于报告基因分析，免疫分析和ATP荧光卫生监测分析；