GLUTAMINASE

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
• in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.