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FREUND'S COMPLETE ADJUVANT

Product NameFREUND'S COMPLETE ADJUVANT
CAS9007-81-2
MFC9H12FN3O5
MW0
EINECS
MOL FileMol File

Chemical Properties

storage temp.	2-8°C
form	cell suspension
color	amber

Safety Information

Safety Statements	23-24/25
WGK Germany	3
RTECS	LS6860000
Storage Class	10 - Combustible liquids
Hazard Classifications	Asp. Tox. 1

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Uses

Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion.

Uses

Immunization

General Description

1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.

Biochem/physiol Actions

The mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts.

in vivo

Complete Freund's adjuvant (CFA) induces experimental autoimmune myocarditis and neuropathic pain, with a relatively low plasma clearance rate (22%)^[1]^[2]^[3]^[4].

Induction of experimental autoimmune myocarditis (EAM)^[1]

Background

Complete Freund's adjuvant (CFA) induces myocarditis by activating the immune system and enhancing the antigen-specific immune response.

Specific Modeling Methods

Mice: A/J, BALB/c, and IL6KO BALB/c male 6-8-week-old
Administration: 5mg/ml s.c. twice

Note

(1) On day 0, all mice additionally received 500 ng of pertussis toxin (i.p.)
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.

Modeling Indicators

Histological analysis: Myocarditis severity was assessed by histology and relative heart weight.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.

Correlated Product(s): Incomplete Freund's adjuvant (IFA) (HY-153808A); Complete Freund's adjuvant (CFA, 1 mg/ml) (HY-153808B)

Opposite Product(s): /

Induction of Neuropathic Pain^[2]^[3]

Background

Complete Freund's adjuvant (CFA) releases inflammatory mediators TNF-α and IL-6 in body which can activate peripheral nerve endings and act on pain nerve endings, resulting in neuropathic pain^[2]^[3].

Specific Modeling Methods

Mice: C57BL/6J male 8-week-old
Administration: 20 μl s.c. once

Note

(1) Inject Complete Freund’s Adjuvant (CFA) without dilution into the plantar area of the hind paw.
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.

Modeling Indicators

Behavior Observation: Detect paw withdrawal threshold (PWT) and paw withdrawal latency (PWL).

Correlated Product(s): Galantamine (HY-76299)

Opposite Product(s): Mecamylamine (HY-B1395A)

Animal Model:	A/J, BALB/c, and IL6KO BALB/c male mice^[1]
Dosage:
Administration:	Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain)
Result:	Increased the relative proportion of CD11b+F4/80monocytes and CD11b+Gr1+ granulocytes. Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6.

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