An efficient method for the isolation of charophyllosides in the following steps:
(1) Take 2 kg of extract of Guangqian with content of about 20% and add 40% ethanol solution to formulate a sample solution of 60 mg/ml;
(2) Place the above sample solution in the crystallization kettle, open the CO2 gas cylinder in the supercritical device, pass CO2 to the crystallization kettle to reach the predetermined temperature 60 ?? and the pressure of 10MPa, turn off the gas cylinder, turn on the regulator to control the balance of the pressure, and the cycle of crystallization for 120min, collect the Crystals I;
(3) Dissolve the above crystals in 40% ethanol solution to form a sample solution of 60mg/ml, and repeat the crystallization 2 times according to the above conditions to obtain crystal III.
(4) The product 227g of 93.3% Xaveratoside can be obtained by high performance liquid chromatography.