Blocky white crystals, soluble in methanol, ethanol, DMSO and other organic solvents, derived from AsarumsieboldiiMiq.
Asatone is an active component isolated from Radix et Rhizoma Asari, with anti-inflammatory effect via activation of NF-κB and donwn regulation of p-MAPK (ERK, JNK and p38) pathways[1].
Asatone, an active ingredient isolated from Asarum, has anti-inflammatory activity by activating NF-κB and downregulating p-MAPK (ERK, JNK and p38) pathways.
Asatone was prepared as follows: air-dried whole powdered air-dried powder (28 kg) of the whole plant of Asarum was extracted with 70% ethanol (3 150 L) under reflux for a total of 6 h, and then the solvent was removed under reduced pressure. The 70% ethanol extract (4360 g) was suspended in water and sequentially partitioned with light petroleum (60-90oC), CHCl3, EtOAc and n-butyl OH. The light petroleum layer (260 g) was subjected to silica gel CC elution and gradient elution with n-hexane-EtOAc (30:1 to 0:1) to give 32 fractions (P-fractions 1-32). Semi-preparative HPLC (85% MeOH in H2O) was performed on P-fraction 6 to give asatone (25 mg).
[1] Chang HY, et al. Asatone Prevents Acute Lung Injury by Reducing Expressions of NF-[Formula: see text]B, MAPK and Inflammatory Cytokines. Am J Chin Med. 2018;46(3):651-671. DOI:
10.1142/S0192415X18500349
