Description
Ac-VEID-AMC is a fluorogenic substrate for caspase-6 and related proteases including caspase-8. Caspase activity can be quantified by fluorescent detection of free AMC (7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.
Uses
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].
References
[1] Benford HL, et al. Visualization of bisphosphonate-induced caspase-3 activity in apoptotic osteoclasts in vitro. Bone. 2001 May;28(5):465-73. DOI:
10.1016/s8756-3282(01)00412-4
Excitation / Emission Maximum
340-360 nm/440-460 nm
Sequene
Ac-Val-Glu-Ile-Asp-AMC
