Biological Activity
This enzyme catalyzes O-glycan branch synthesis of the core 2 and core 4 type in mucins and controls expression of core 2 branched oligosaccharides and I antigens on the cell surface.This enzyme catalyzes O-glycan branch synthesis of the core 2 and core 4 type in mucins and controls expression of core 2 branched oligosaccharides and I antigens on the cell surface.
Synthesis
1-Naphthylamine was added to the reactor and melted by heating, then dehydrated at 110C-180C for 2-3 hours. Add aniline and p-aminobenzenesulfonic acid, stir well, then slowly raise the temperature to 230-240 in 25 hours for condensation reaction. The ammonia released in the reaction is absorbed with water and sulfuric acid to produce ammonia and ammonium sulfate by-products. When the amount of ammonia released in the reaction is very small, the reaction is stopped, neutralized to neutral by adding soda ash, and fractionated in high vacuum. Recover unreacted aniline and naphthylamine, and vaporize the product. After cooling, slicing and packing, it is N-phenyl-1-naphthylamine hydrobromide.
