Chemical Properties
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Uses
These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
Definition
ChEBI: A methylguanine in which the methyl group is positioned on the oxygen at position 6. Formed in DNA by alkylation of the oxygen atom of guanine, most often by N-nitroso compounds and sometimes due to methylation by other compounds such as
endogenous S-adenosylmethionine, it base-pairs to thymine rather than cytidine, causing a G:C to A:T transition in DNA.
Application
6-O-Methyl-guanine is a key molecular tool for studying DNA damage, repair mechanisms, and cancer chemotherapy. It is a potent mutagenic and carcinogenic DNA damage product induced by alkylating agents (such as certain environmental carcinogens and chemotherapeutic drugs). It can mispair with thymine (T), leading to G:C to A:T base transition mutations during DNA replication. Therefore, O⁶-MeG is widely used in in vitro and cellular experiments to simulate DNA damage states and study how cells remove this damage through repair proteins such as O⁶-methylguanine-DNA methyltransferase (MGMT).
General Description
Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards
