HEPPS Struktur

4-(2-Hydroxyethyl)-1-piperazinpro-pansulfonsäure

Bezeichnung:4-(2-Hydroxyethyl)-1-piperazinpro-pansulfonsäure

CAS-Nr16052-06-5

Englisch Name:HEPPS

CBNumberCB9310937

SummenformelC9H20N2O4S

Molgewicht252.33

MOL-Datei16052-06-5.mol

Synonyma

4-(2-Hydroxyethyl)-1-piperazinpropansulfonsure

4-(2-Hydroxyethyl)-1-piperazinpro-pansulfonsäure

4-(2-Hydroxyethyl)-1-piperazinpro-pansulfonsäure physikalisch-chemischer Eigenschaften

Schmelzpunkt	237-239 °C(lit.)
Dichte	1.2684 (rough estimate)
Brechungsindex	1.6800 (estimate)
storage temp.	Sealed in dry,Room Temperature
Löslichkeit	H₂O: 1 M at 20 °C, clear, colorless
Aggregatzustand	Powder/Solid
Farbe	White
PH	5.0-6.5 (25℃, 0.1M in H2O)
pka	8.0(at 25℃)
Säure-Base-Indikators(pH-Indikatoren)	7.3 - 8.7
Wasserlöslichkeit	Soluble in water. 25.2 g/L (20°C)
maximale Wellenlänge (λmax)	λ: 260 nm Amax: 0.055 λ: 280 nm Amax: 0.045
BRN	3957385
CAS Datenbank	16052-06-5(CAS DataBase Reference)
EPA chemische Informationen	1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38-22
S-Sätze:	24/25-36-26
WGK Germany	3
RTECS-Nr.	TM2649130
TSCA	Yes
HazardClass	IRRITANT
HS Code	29335995
Toxizität	quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982.

Gefahreninformationscode (GHS)

HEPPS Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Chemische Eigenschaften
white crystalline powder
Verwenden
HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.
Definition
ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine.
Allgemeine Beschreibung
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.
Flammability and Explosibility
Not classified

4-(2-Hydroxyethyl)-1-piperazinpro-pansulfonsäure Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

16052-06-5, HEPPS Verwandte Suche:

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