4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure

Bezeichnung:4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure

CAS-Nr7365-45-9

Englisch Name:HEPES

CBNumberCB5408557

SummenformelC8H18N2O4S

Molgewicht238.3

MOL-Datei7365-45-9.mol

Synonyma

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure physikalisch-chemischer Eigenschaften

Schmelzpunkt	234-238 °C
Siedepunkt	408℃[at 101 325 Pa]
Dichte	1.07 g/mL at 20 °C
Dampfdruck	0Pa at 25℃
Brechungsindex	n20/D 1.339
storage temp.	2-8°C
Löslichkeit	H₂O: 1 M at 20 °C, clear, colorless
Aggregatzustand	powder
Farbe	White
PH	5.0-6.5 (1Maqueous solution)
pka	7.5(at 25℃)
Säure-Base-Indikators(pH-Indikatoren)	7.0 - 7.6
Wasserlöslichkeit	Soluble
maximale Wellenlänge (λmax)	λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04
Merck	14,4654
BRN	883043
Stabilität	Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey	JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP	-3.85 at 20℃
CAS Datenbank	7365-45-9(CAS DataBase Reference)
EPA chemische Informationen	1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	24/25-22-36-26
WGK Germany	1
RTECS-Nr.	TL6809000
TSCA	Yes
HS Code	29335995

Gefahreninformationscode (GHS)

HEPES Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemische Eigenschaften
White crystalline powder
Verwenden
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Verwenden
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Verwenden
HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.
Definition
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
Allgemeine Beschreibung
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biologische Aktivität
Multi purpose buffer used in biological research.
Biologische Aktivität
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Lager
Room temperature
läuterung methode
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

HEPES Upstream-Materialien And Downstream Produkte

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

7365-45-9, HEPES Verwandte Suche:

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