3-Methyl-3H-adenin

Bezeichnung:3-Methyl-3H-adenin

CAS-Nr5142-23-4

Englisch Name:3-METHYLADENINE

CBNumberCB9307216

SummenformelC6H7N5

Molgewicht149.15

MOL-Datei5142-23-4.mol

Synonyma

3-Methyl-3H-adenin

3-Methyl-3H-adenin physikalisch-chemischer Eigenschaften

Schmelzpunkt	~300 °C (dec.)(lit.)
Siedepunkt	240℃
Dichte	1.60
Brechungsindex	1.8070 (estimate)
Flammpunkt	99℃
storage temp.	Keep in dark place,Sealed in dry,Room Temperature
Löslichkeit	Soluble in DMSO (up to 3 mg/ml), or in DMF (up to 10 mg/ml).
pka	6.77±0.20(Predicted)
Aggregatzustand	Powder
Farbe	White
BRN	146087
Stabilität	Stable for 2 years from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20° for up to 3 months.

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	22
S-Sätze:	24/25
WGK Germany	3
RTECS-Nr.	AU6520000
HS Code	29335990

Gefahreninformationscode (GHS)

3-METHYLADENINE Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R22:Gesundheitsschädlich beim Verschlucken.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
Beschreibung
Autophagy is a multi-step process that involves the degradation and digestion of intracellular components by the lysosome. This process allows cells to efficiently mobilize and recycle cellular constituents, and also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms. mTOR, whose activation is controlled by phosphoinositide 3-kinase (PI3K), is a key regulator of autophagy. 3-Methyladenine (3-MA) is a specific inhibitor of PI3K activity and one of the most widely used inhibitors of the initial phase of the autophagic process: the sequestering of cytoplasmic material by the lysosome. At 5 mM, 3-MA inhibits protein degradation in rat hepatocytes by 65%. 3-MA has been shown to block class I, class II, and class III PI3Ks, including some downstream targets, and to suppress the invasion of highly metastatic human fibrosarcoma HT1080 cells at 10 mM.
Chemische Eigenschaften
White powder
Verwenden
A synthetic intermediate and a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis post serum/potassium deprivation
Verwenden
A multi-use inhibitor that protects cerebellar granule cells from apoptosis.
Verwenden
3-Methyladenine (3-MA) inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.
Definition
ChEBI: A methyladenine that is adenine substituted with a methyl group at position N-3.
Allgemeine Beschreibung
A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC₅₀ = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
Biochem/physiol Actions
3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).
Lager
+4°C
Einzelnachweise
1) Seglen and Gordon?et al.?(1982),?3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes; Proc. Natl. Acad. Sci. USA,?79?1889 2) Blommaart?et al. (1997),?The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes; Eur. J. Biochem.,?243?240 3) McFarland?et al. (2012),?Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity; Arch. Toxicol.,?86?275 4) Zhao?et al. (2014),?Inhibition of autophagy strengthens celastrol-induced apoptosis in human pancreatic cancer in vitro and in vivo models; Curr. Mol. Med.,?14?555

3-METHYLADENINE Upstream-Materialien And Downstream Produkte

3-Methyl-3H-adenin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

5142-23-4, 3-METHYLADENINE Verwandte Suche:

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