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5704-04-1

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Identification

Name
Tricine
CAS
5704-04-1
Synonyms
2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL, HYDROCHLORIDE
LABOTEST-BB LT00454966
MOLE-READY-TRIS
N-[2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]GLYCINE
N-[TRIS(HYDROXYMETHYL)METHYL]GLYCINE
SPECTRIS(R) HYDROCHLORIDE
SPECTRIS(TM) HYDROCHLORIDE
TRICINE
TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE
TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE
TRIS-HCL
TRIS-HCL BUFFER
TRIS HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE, HCL
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE BUFFER SOLUTION
TRIS(HYDROXYMETHYL)METHYLAMINE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)METHYLAMMONIUM CHLORIDE
TRIS(HYDROXYMETHYL) METHYLGLYCINE
TRISMAT(R) HYDROCHLORIDE
EINECS(EC#)
214-684-5
Molecular Formula
C6H13NO5
MDL Number
MFCD00012590
Molecular Weight
179.17
MOL File
5704-04-1.mol

Chemical Properties

Appearance
White/clear crystalline powder
Melting point 
186-188 °C (dec.)

mp 
186-188 °C (dec.)

Boiling point 
311.69°C (rough estimate)
density 
1.05 g/mL at 20 °C

refractive index 
1.4240 (estimate)
storage temp. 
Store at RT.
solubility 
H2O: 4 M at 20 °C, clear, colorless

form 
crystalline

color 
clear colorless (40 % (w/w) in H2O) solution

Odor
Odorless
Stability:
Stable.
PH
4.0-6.0 (1M in H2O)
PH Range
7.4 - 8.8
pka
8.1(at 25℃)
Water Solubility 
soluble
Merck 
14,9651
BRN 
1937804
CAS DataBase Reference
5704-04-1(CAS DataBase Reference)
EPA Substance Registry System
5704-04-1(EPA Substance)

Safety Data

Hazard Codes 
Xi
Risk Statements 
R36/37/38:Irritating to eyes, respiratory system and skin .
Safety Statements 
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
WGK Germany 
3


3
TSCA 
Yes
HS Code 
29052900

Questions And Answer

Descriptioin
Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
Reference
  1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
  2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379

Hazard Information

Chemical Properties
White/clear crystalline powder
Uses
Biological buffer.
Purification Methods
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

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