Dipotassium hydrogen phosphate trihydrate (K2HPO4•3H2O) is the dipotassium form of phosphoric acid, which can be highly soluble in water. It is widely applied in the fields of food, agriculture and molecular biology etc. It is commonly used as a food additive, involved in the formation of dairy creamers, dry powder beverages. In addition, as a source of phosphorus and potassium, it is allowed to be used as a electrolyte replenisher, providing minerals especially for athletes, which play an essential role in the recovery of muscles. It can also serves as a fertilizer to increase in rice grain yield. Besides, it has application in molecular biology and biochemistry, which is useful to increase the partition coefficients of amino acids in aqueous two-phase systems. Besides, It has been proved that Dipotassium hydrogen phosphate has radio-protective activity, which is able to block the uptake of the radioactive isotope P-32 upon oral administration.