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Нитро синий тетразолий хлорид
- английское имяNitrotetrazolium blue chloride
- CAS №298-83-9
- CBNumberCB8465489
- ФормулаC40H30ClN10O6+
- мольный вес782.19
- EINECS206-067-4
- номер MDLMFCD00012159
- файл Mol298-83-9.mol
Температура плавления | 200°C |
плотность | 1.5521 (rough estimate) |
показатель преломления | 1.7350 (estimate) |
температура хранения | 2-8°C |
растворимость | methanol: 50 mg/mL, clear, very strongly yellow |
форма | Powder/Solid |
цвет | Yellow |
Растворимость в воде | Soluble in water, ethanol and methanol. |
Чувствительный | Light Sensitive |
λмакс | 256 nm |
БРН | 4115923 |
Стабильность | Stable, but store cold. |
Биологические приложения | Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting |
FDA UNII | X44P41F7ZK |
Система регистрации веществ EPA | 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9) |
Коды опасности | Xn,Xi | |||||||||
Заявления о рисках | 22-68/20/21/22-20/21/22-36-36/37/38-41 | |||||||||
Заявления о безопасности | 36-45-36/37-26-39 | |||||||||
WGK Германия | 3 | |||||||||
RTECS | XF8045000 | |||||||||
F | 8-10-23 | |||||||||
TSCA | Yes | |||||||||
кода HS | 32041900 | |||||||||
Токсичность | LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990 | |||||||||
NFPA 704: |
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рисовальное письмо(GHS)
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рисовальное письмо(GHS)
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сигнальный язык
опасность
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вредная бумага
H315:При попадании на кожу вызывает раздражение.
H319:При попадании в глаза вызывает выраженное раздражение.
H360D:Может отрицательно повлиять на неродившегося ребенка.
H332:Вредно при вдыхании.
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оператор предупредительных мер
P201:Беречь от тепла, горячих поверхностей, искр, открытого огня и других источников воспламенения. Не курить.
P261:Избегать вдыхания пыли/ дыма/ газа/ тумана/ паров/ аэрозолей.
P264:После работы тщательно вымыть кожу.
P280:Использовать перчатки/ средства защиты глаз/ лица.
P304+P340+P312:ПРИ ВДЫХАНИИ: Свежий воздух, покой. Обратиться за медицинской помощью при плохом самочувствии.
P308+P313:ПРИ подозрении на возможность воздействия обратиться за медицинской помощью.
Нитро синий тетразолий хлорид химические свойства, назначение, производство
Описание
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.Химические свойства
NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.Использование
Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.прикладной
Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.
Определение
ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).Общее описание
Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.Опасность
Moderately toxic by ingestion.Биохимия/физиол Действия
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.Профиль безопасности
Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.Нитро синий тетразолий хлорид запасные части и сырье
Нитро синий тетразолий хлорид поставщик
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