N,N-Bis(4-sulfobutyl)-3,5-dimethylaniline disodium salt

N,N-Bis(4-sulfobutyl)-3,5-dimethylaniline disodium salt (MADB) is an important colour-forming reagent used in the quantitative detection of glucose. The function of MADB is to undergo an oxidative coupling reaction with 4-aminoantipyrine (4-AAP) in the presence of hydrogen peroxide and horseradish peroxidase (HRP), producing a coloured oxidised complex ^[1–3].

[1] Jang, H., Oh, J., Ki, H., Kim, M.-G. (2020). Paper-based 1,5-anhydroglucitol quantification using enzyme-based glucose elimination†. Analyst, 17, 5740–5743. https://doi.org/10.1039/D0AN00905A
[2] Gyeo-Re Han. (2021). Reagent Filming for Universal Point-of-Care Diagnostics. Small Methods, 5 12. https://doi.org/10.1002/smtd.202100645
[3] Jie Cheng, Jinhong G., Jiuchuan Guo. (2023). A Low-cost Creatinine Biosensor by Differential Optical Signal Readout for the Whole Blood Analysis. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2023, 1–4. https://doi.org/10.1109/EMBC40787.2023.10341195