5-CARBOXYFLUORESCEIN DIACETATE

Chemical Properties

Melting point	>250°C
Boiling point	693.1±55.0 °C(Predicted)
Density	1.57±0.1 g/cm3(Predicted)
storage temp.	Sealed in dry,Store in freezer, under -20°C
solubility	DMSO: soluble
pka	6.4(at 25℃)
form	Solid
color	Colorless solid
PH Range	Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
λmax	<300nm
BRN	68265
Biological Applications	Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity
Major Application	Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs