Basic information Safety Related Supplier

ChemicalBook Product Catalog 5-CFDA

1/1

5-CFDA NEW

$33.00 25mg

$47.00 50mg

Min. Order
Purity98.39%
Cas No79955-27-4
Supply Ability10g
Update time2026-01-26

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP4Y

United States

United States

show more

Enterprise Verified

Business Bank account Basic Contact Infomation Business Address Trade Company

Chemical Properties

Product Name	5-CFDA
CAS No	79955-27-4
EC-No
Min. Order
Purity	98.39%
Supply Ability	10g
Release date	2026/01/26

Product Introduction

Bioactivity

Name	5-CFDA
Description	5-CFDA (5-Carboxyfluorescein diacetate)(5-Carboxyfluorescein diacetate) is membrane-permeant. It can be loaded into cells via incubation and hydrolyzed by intracellular esterases to 5-carboxyfluorescein that is used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.
Cell Research	Instructions I. Solution preparation 1. Stock solution: Dissolve 5-CFDA in anhydrous DMSO to prepare a stock solution with a concentration of 1–10 mM. Note: Keep away from light during operation. Store the stock solution at -20°C after aliquoting. Avoid repeated freezing and thawing to ensure stability. 2. Working solution: Dilute the stock solution to the final use concentration (1–10 µM). Phenol red-free cell culture medium or PBS buffer can be used for dilution. II. Operation steps 1. Cell preparation: Collect the adherent or suspension cells to be treated according to the experimental requirements and adjust to the appropriate cell density; rinse the cells 1–2 times with PBS or phenol red-free culture medium to remove substances that may interfere with the experiment. 2. Staining reaction: Add the prepared 5-CFDA working solution to the cell suspension or culture medium to ensure that the dye is in full contact with the cells: usually at a concentration of 1–10 µM, incubate at 37°C in the dark for 15–30 minutes. 3. Washing steps: Wash the cells 2–3 times with PBS or fresh culture medium to remove excess dye that is not absorbed by the cells. 4. Detection and result analysis 1) Fluorescence signal detection: Detection is performed using a fluorescence microscope, flow cytometer, or fluorescence plate reader. Recommended parameters are: Excitation wavelength: 490–495 nm Emission wavelength: 515–525 nm 2) Data processing: Compare the fluorescence intensity of the experimental group and the control group to evaluate cell activity, track cells, or analyze other biological dynamic changes.
Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	10% DMSO+40% PEG300+5% Tween-80+45% Saline : 3.3 mg/mL (7.17 mM), Sonication is recommeded. DMSO : 100 mg/mL (217.21 mM), Sonication is recommended.
Keywords	Inhibitor \| inhibit \| 5-CFDA \| 5CFDA \| 5-Carboxyfluorescein Diacetate \| 5-Carboxyfluorescein \| 5 CFDA
Related Compound Libraries	Human Metabolite Library

show more

Company Profile Introduction

You may like

Recommended supplier

MSDS|CAS|CAS DataBase|Pricacy|Terms|About Us|New Products| Contact us|Computer Version
Chemical Book