Acetic acid-d4

Malonic acid-d2-acid-d2 (2.7 g, 25 mmol) was melted at 140.MALE.C, and then water (112.5 μL, 6.25 mmol) was added with stirring. The mixture was heated to 150.MALE.C. After 15 minutes, a colorless liquid (1.32 g, 85%) was collected by distillation at atmospheric pressure. The product was stirred with 0.2 mL of D2O, the water was pumped out, and the sample was dried under vacuum with a cold trap. The product was further purified by fractional distillation at atmospheric pressure in a closed drying system to give 0.61 g of product. ¹H NMR of the product revealed three signals with integrals of 3.6%: 31.2%: 65.2%, corresponding to acetic acid:acetic acid-acetic acid:acetic acid-2-acid.

¹H NMR (500 MHz, D₂O) ≠ 1.95 (s, CH₃), 1.93 (t, J = 2.5 Hz, CDH₂), 1.92 (q, J = 2.0 Hz, CD₂H). Since the D:H ratio in the reaction mixture is 8:1, the product also contains >50% acetic acid-d₃, which is not visible in ¹H NMR. ¹³C NMR (D₂O) ≠ 176.5, 20.2 (quintet).

colourless liquid

Acetic acid-d₄ may be used in the synthesis of deuterated (7E,9Z)-CLA (conjugated linoleic acid) and Pr(CD₃COO)₃.D₂O (deuterium analog of praseodymium (III) acetate hydrate).

Acetic acid-d{4} is used as a deuterated, polar and protic solvent, in food preservatives, PH control agent, flavor enhancer.

(Acetic acid-D4) Labelled Acetic Acid is used for analysis and profiling of biological samples resolved through NMR spectroscopy. It helps with the labelling of RNA for high resolution NMR.

ChEBI: Acetic acid-d4 is a deuterated compound and a monocarboxylic acid.

Acetic acid-d₄ (deuterated acetic acid) is a deuterated NMR solvent containing 0.03% (v/v) TMS (tetramethylsilane). It is useful in NMR-based research and analyses. Its dissociation constant in deuterium oxide has been obtained over a range of temperature by emf method.