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Fluorescamine

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Fluorescamine Basic information
Fluorescamine Chemical Properties
  • Melting point:153-157 °C(lit.)
  • Boiling point:381.08°C (rough estimate)
  • Density 1.2661 (rough estimate)
  • refractive index 1.5447 (estimate)
  • Flash point:-17 °C
  • storage temp. 2-8°C
  • solubility acetonitrile: soluble
  • form powder
  • color off-white to yellow
  • Water Solubility Soluble in acetone, ethanol, chloroform, dimethylsulfoxide and acetonitrile. Slightly soluble in water.
  • Merck 14,4158
  • BRN 921143
  • Stability:Stable. Incompatible with strong oxidizing agents.
  • CAS DataBase Reference38183-12-9(CAS DataBase Reference)
  • NIST Chemistry ReferenceSpiro[furan-2(3h),1'(3'h)-isobenzofuran]-3,3'-dione, 4-phenyl-(38183-12-9)
Safety Information
MSDS
Fluorescamine Usage And Synthesis
  • Chemical Propertieswhite powder
  • UsesFluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis.
  • UsesAnalytical reagent.
  • Purification MethodsFluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3
Fluorescamine Preparation Products And Raw materials
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