2',7'-二氯荧光素二乙酸酯
2',7'-二氯荧光素二乙酸酯 性质
| 熔点 | 209-210 °C(lit.) |
|---|---|
| 沸点 | 593.5±50.0 °C(Predicted) |
| 密度 | 1.463±0.06 g/cm3(Predicted) |
| 储存条件 | -20°C |
| 溶解度 | 在DMF中可溶 |
| 酸度系数(pKa) | 3.79±0.36(Predicted) |
| 形态 | 结晶固体 |
| 颜色 | 白色至米白色 |
| BRN | 4610273 |
| 稳定性 | 感光 |
| EPA化学物质信息 | 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0) |
2',7'-二氯荧光素二乙酸酯 用途与合成方法
2',7'-二氯荧光素二乙酸酯是一种荧光探针，作为一种非极性化合物，能够迅速扩散到细胞内，细胞内的酯酶将其水解为非荧光的二氯荧光素（DCFH），从而使探针被装载到细胞内。
H2DCFDA (DCFH-DA, 2',7'-Dichlorodihydrofluorescein diacetate) 是一种可渗透细胞的探针，可用于检测细胞内 reactive oxygen species (ROS) 的产生。| Target | Value |
|
ROS
() |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
2',7'-二氯荧光素二乙酸酯 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-11 | XW40919902 | 2-(3,6-二乙酰氧基-2,7-二氯-9H-氧杂蒽-9-基)苯甲酸 | 4091-99-0 | 10MG | 102 |
| 2024-11-11 | XW40919901 | 2-(3,6-二乙酰氧基-2,7-二氯-9H-氧杂蒽-9-基)苯甲酸 | 4091-99-0 | 50MG | 259 |
