EC 1.4.3.14

Product NameEC 1.4.3.14
CAS70132-14-8
MF
MW0
EINECS
MOL FileMol File

Chemical Properties

storage temp.	2-8°C
form	lyophilized powder
biological source	fungus (Trichoderma viride)
Specific Activity	≥20units/mg protein

Safety Information

WGK Germany

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Uses

Lysine Oxidase from Trichoderma viride has been used in the preparation of luminescent biochip preparation.

General Description

Lysine Oxidase from Trichoderma viride is a homodimeric flavoenzyme corresponding to molecular mass of 112 kDa. It is stable at 65°C and is highly specific for L-lysine substrate. It comprises FAD-binding, substrate binding and a helical domain with distinct active site funnel.

Biochem/physiol Actions

Lysine Oxidase from Trichoderma viride catalyzes the formation of α-keto- ε-aminocaproate by the oxidative deamination of L-lysine. It displays anti-tumor functionality in cancer leukaemic cells. It is a tumor suppressor for squamous cell, fibroblast, ovarian and gastric tumors. Lysine oxidase also plays key role in connective tissue structural integrity and embryo development.

in vivo

L-Lysine α-oxidase (150 U/kg on day 2 and 75 U/kg on days 4, 6, 8, and 10; i.p.) shows anticancer activity in human tumor models^[2].

Animal Model:	6-9 weeks, Female Balb/c nude mice (SKBR3, HCT116, Bro, SKOV3, Alex, T47D, LS174T tumor models)^[2]
Dosage:	150 U/kg on day 2 and 75 U/kg on days 4, 6, 8, and 10
Administration:	I.p.; 10 U/ml saline solution
Result:	Caused inhibition of tumor growth with maximal T/C of 49, 12, 51, 35, 54,36, 37% for SKBR3, HCT116, Bro, SKOV3, Alex, T47D, LS174T tumor models, respectively.